Originally, I thought Seed Line. But
@NiceMarmot set me straight that it still goes off geography at the regional level (just like the first weekend is) as long the overall 5 seed isn’t with the overall 1 seed and all other rules remain in place (no other 4-seed or better teams from same conference in the same region, etc.).
We can’t go to the South since Houston will be the 1 there. Since, Boston is closer to Ames that would be our preference versus LA (even though it really isn’t). So our only hope to avoid the East region would be to leapfrog Marquette to get preference for Detroit over the Golden Eagles.